In my many years of biohacking and using nootropics to optimize my brain, I have found basic blood work is fundamental to measuring my success. Lab tests give me a more accurate window into what these supplements are doing to my body and brain. More so than simply relying on how I feel.
If you’ve been using nootropics for an length of time, you may have wondered how to go further in reaching your biohacking goals. Blood tests and other lab work can go a long way in helping refine your nootropic stack.
Doing blood work on a regular basis, even when you’re not sick, gives you the ability to find out if you’re going in the right direction. Nootropics affect more than just your brain. Supplements affect the rest of your body too.
Basic blood work will tell you about hormones, inflammation, vitamin and minerals levels, and how your organs and glands are doing. And you can eliminate the guesswork by getting real data about your body.
If something shows up that’s out of range, you can easily take steps to correct it. Often by simply modifying your nootropic stack. You can take a proactive approach and prevent major problems from ever happening.
This post is about types of lab work available. And what types of tests you may want to consider.
Blood Tests for Neurohackers
For neurohackers, information provided by blood tests are vital to optimizing both mental and physical performance. You get accurate data on specific biochemistry. And how your body is responding to changes in diet, lifestyle, and the nootropic supplements you’re taking.
Basic bloodwork will give you a window into:
- Blood health
- Thyroid health
- Vitamin & mineral levels
- Adrenal gland, kidney and liver health
- Hormone health
There is the temptation, especially when you’re early in your neurohacking journey, to go after specialized tests. Like having your neurotransmitters tested. We’ll cover those next.
While the big, basic tests may not be nearly as exciting, they tell you volumes about your body. And overall health. Especially cognitive health.
Our primary goal in brain optimization typically has to do with altering brain chemistry. In fact, studies estimate that 86% of Americans have low neurotransmitter levels.[i]
Sub-optimal neurotransmitter levels are caused by stress, poor diet, neurotoxins, faulty genes, prescription and recreational drugs, alcohol and caffeine.[ii] Sounds like most of us. Right?
So it seems reasonable that we could get a more accurate picture on what’s going on in our brain if we get our neurotransmitter levels tested.
It turns out that most neurotransmitter lab tests are useless. And here’s why…
The least expensive and most common lab test advertised is neurotransmitter urine testing. The problem is that there is nearly zero correlation between neurotransmitter levels in the brain and the rest of your body.
The neurotransmitters in your brain are primarily synthesized in the neurons in your brain. These brain chemicals never make it to your kidneys for urine output.
It’s absolutely true that this serotonin affects anxiety, depression and mood. But this brain-gut connection (which I’ve written about earlier here) cannot be accurately measured by reading levels of serotonin in your urine. Because much of this serotonin goes directly to your kidneys and is excreted in urine.
The microbiome in your gut manufactures more than 30 neurotransmitters. Including serotonin. Which take care of other bodily functions. But also send messages to your brain. Affecting anxiety, stress, learning, memory, decision-making and depression.[iv]
Measuring neurotransmitter levels in your urine from neurochemicals synthesized in your gut give no indication on what’s going on in your brain.[v]
Another problem with urine testing is the half-life of many of the main neurotransmitter molecules. Serotonin has a half-life of one second.[vi] Others a fraction of a second.
Then you may have issues with damaged, depleted or non-existent neuroreceptors. Or other compounds bound to these receptors rather than the intended neurotransmitter.
One final note on neurotransmitter urine testing. Research has verified that urinary neurotransmitter testing is not an accurate way to test levels in your brain.[vii] And saliva neurotransmitter tests are as unreliable as urine testing.
The only method left is serum or blood testing for neurotransmitters. While this is considered more accurate than urine or saliva testing, it still doesn’t tell us much. Because your neurotransmitter levels vary by the hour and by the day.
But all is not lost because we have other ways to measure our biohacking progress in the lab.
Most Important Blood Tests for Neurohacking
Those of us interested in neuro- and biohacking have found that a basic battery of tests performed at least annually give us a clear picture of our physical and psychological health.
Here we’ll take a brief look at the most common blood tests. And why they matter to your neurohacking success.
CBC Panel (Chemistry & Complete Blood Count)
This panel tells you about the presence of anemia, infectious organisms, nutritional deficiencies, lymphatic issues, and blood disorders.
For example, when something is wrong with your blood. It will affect your total health. Including brain function. Anemia will cause weakness, fatigue, dizziness and headache.
A problem with your kidneys can show up as low (or high) sodium, potassium and chloride ions. Ion channels and their associated ions directly influence how each of your neurons communicate and use neurotransmitters.
Abnormally low RBC levels could indicate deficiencies in iron, folate, Vitamin B6 and B12. The last three are directly tied to synthesis of most of your major neurotransmitters. And easily corrected with the use of nootropic supplements.
Increased levels of WBCs (lymphocytes, monocytes, neutrophils, eosinophils, and basophils) are typically associated with bacterial, viral, parasitic or protozoal infections. Supplementing with Iodine, St. John’s wort, or Mucuna Pruriens (L-Dopa) can kill bacteria, fungal infections and viruses.
DHEA measures your adrenal function. Low levels of DHEA show up as memory loss, depression, Alzheimer’s, chronic inflammation and other problems. Adding DHEA to your nootropic stack may be all you need to improve memory and mood.
This hormone level check is particularly important for aging men, and women after menopause. In men, increased levels of estradiol and decreased levels of testosterone usually indicate low libido and difficulty urinating. Other symptoms include excess fat, brain fog, depression and fatigue.
Excess homocysteine is an important risk factor for heart disease and stroke. Research shows that elevated homocysteine levels in those with depression are a result of too little folic acid.[viii] Adding Vitamin B9 (Folate) and Vitamin B12 to your stack may be all you need to improve mood and bring homocysteine levels down.
C-reactive protein (CRP)
C-reactive protein is a very powerful indicator of inflammation both in your body and brain.
Elevated levels of C-reactive protein are implicated in developing type II diabetes,[ix] loss of information processing, recall, and memory ability (even in healthy people),[x] Alzheimer’s and depression.
TNF-a, IL-6, IL-1b and IL-8
Proinflammatory cytokines tumor necrosis factor-a (TNF-a), interleukin-1 beta (IL-1b), interleukin-6 (IL-6), and interleukin-8 (IL-8) regulate C-reactive protein. And knowing them may help identify the cause of inflammation.
These cytokines are cellular growth factors that are synthesized in nearly every cell in your body. Generally only in response to stress. There is growing evidence that depression could stimulate proinflammatory IL-6.[xi]
Excess proinflammatory cytokines can cause severe inflammation leading to diseases like Alzheimer’s and Parkinson’s. They can deplete neurotransmitters which leads to cognitive decline. And excess cytokines can result in brain fog, anxiety, depression, fatigue, and overall poor mental performance.
Adding Artichoke Extract (Luteolin), Cat’s Claw, Ginseng, L-Glutamine, Oat Straw, SAM-e, Vitamin B3 (Niacin), Vitamin B6 (P-5-P), Vitamin B8 (Inositol), or N-Acetyl L-Cysteine (NAC) to your nootropic stack can keep proinflammatory cytokines under control.
But make sure you review each nootropic article because certain nootropics may only control one or two of these inflammatory cytokines. Find out which ones work for what’s indicated in your blood work.
Folate and Vitamin B12
Deficiencies in both vitamins are due to poor absorption in the gut, pancreatitis, tapeworm, or high alcohol consumption to name a few. Vitamin B12 can only be obtained from food through animal sources. So is a problem for vegetarians and vegans.
Folate deficiency is common in those with problems with the MTHFR gene. This enzyme is needed to convert folic acid and food folate into 5-methylenetetrahydrofolate that your body can use. Up to 60% of people have issues with the MTHFR gene.
Both of these vitamins are easy to check with a blood test. And lacking in either of these vitamins reduces the efficacy of your entire nootropic stack. For a few cents a day you can support the investment you’ve made in your daily stack.
Fasting serum insulin is used to measure insulin sensitivity and resistance. Insulin resistance in our modern world is affecting people of every age group.
One study was done at the University of Michigan in 2006 with 4,902 people aged 12 – 19. Weight status was the largest indicator of who was going to be insulin resistant. In this study, the prevalence of insulin resistance in obese kids was 52.1%.[xii]
Insulin resistance often results in anxiety and depression. One study showed that it altered metabolism in mitochondria. The scientists found that mitochondria increased the production of two enzymes that degraded dopamine. So dopamine was metabolized faster and didn’t last as long in the brain. Resulting in anxiety and depression.[xiii]
We know that insulin helps control levels of blood sugar by helping cells to take in sugar through cellular membranes. And insulin secretion is regulated by hormones in the hypothalamus region of your brain.
Researchers are just recently discovering that specific insulin receptors in regions of the brain have different affects. For example, insulin receptors in the hippocampus are involved in learning and memory.
For now, I highly recommend you get your fasting insulin checked if you’re serious about brain optimization. Find a good naturopathic doctor to help you interpret the results. And provide recommendations on how to reverse insulin resistance.
IGF-1 (insulin-like growth factor)
Insulin-like growth factors (IGF-1 and IGF-2) are proteins, and their receptors, are part of a complex system used to help cells in the central nervous system communicate.
IGF-1 are involved in neuron survival, neurogenesis, excitatory and inhibitory neurotransmission, regulation of food intake and cognition. Recent research has found a link between brain insulin/IGF-1 and certain neurodegenerative diseases including Alzheimer’s.[xiv]
Thyroid (TSH, T4, T3 and free T3)
Thyroid stimulating hormone (TSH) is secreted by your pituitary gland which controls thyroid hormone secretion by your thyroid. Your thyroid then releases thyroxine (T4) and triiodothyronine (T3, free).
Iodine is the essential trace element that combines with the amino acid tyrosine to form thyroid hormones T4 and T3. Thyroxine (T4) contains four iodine atoms, and triiodothyronine (T3) contains three iodine atoms.
The problem is unless you have symptoms for hypo- or hyperthyroidism, it’s unlikely that your doctor will order a thyroid panel for you. Unless you ask them specifically.
Most doctors are trained and don’t know any better than to simply order a TSH blood test. Measuring TSH is not a reliable way to assess the health of your thyroid.
Why is this important? You could be dealing with thyroid problems and not be aware of it until symptoms get severe. In my case, I ended up in the ER with what I thought was a heart attack. Turns out I was hypothyroid.
Thyroid problems can be caused by a host of things. But the most common is an Iodine deficiency. Insufficient iodine is not only a problem in developing countries. Studies have found this holds even in Western countries; iodine deficiency has become a critical health problem.[xvi]
This is especially critical for anyone working on optimizing your brain. Within your brain, T4 is converted to T3 by selenium which then affects gene expression controlling metabolism within cells. And activates the catecholamines dopamine, norepinephrine and epinephrine.
A malfunctioning thyroid caused by iodine deficiency results in brain fog, poor cognition, difficulty learning, problems with recall, depression and anxiety. All symptoms I personally experienced until I got my thyroid levels back in line.
It would take pages to explain the importance of correctly reading a Thyroid Panel. If you have a feeling for any reason that you could have thyroid issues, I highly recommend Janie Bowthorpe’s book “Stop The Thyroid Madness”.
As a neurohacker, you have several options for maintaining thyroid health. By far the easiest thing you can do is add Iodine to your nootropic stack.
Other Blood Tests
Labs for testosterone, estradiol and other sex hormones in men, and testosterone and estrogen in women are also important to check. And all have an influence to varying degrees on cognitive function.
Low testosterone in men and women for example can have a profound effect on anxiety, depression, and memory.[xvii] While nootropics cannot directly raise levels of things like testosterone, you need to be aware of their importance for brain optimization.
Reading Lab Results
Traditionally trained doctors accept imbalances in many key blood tests as part of natural aging. Their only concern is when your result may be outside of the normal lab “reference range”.
A perfect example of this is for men who typically suffer with excess production of insulin and estrogen. Along with simultaneous deficiencies in testosterone and DHEA. And this is happening to younger and younger men.
If your regular doctor used the standard “reference range” and your labs came back at the low end of the standard range. You would not feel well. But your doctor would declare nothing wrong with you.
The same thing is happening to both men and women with thyroid testing. A recent study at Stobhill Hospital in Glasgow found that patients who were hypothyroid were considered within “normal” TSH reference ranges. But they were “psychologically impaired”.[xviii]
I learned this through much trial an error, a few years of feeling like crap, firing no less than six endocrinologists and a few doctors. This led me to doing my own research and learning how to read my own lab results.
I encourage you to do the same. Find out exactly what is going on in your body and brain with the tools you have available to you.
Learn to read your own labs. Because if you don’t know what to do with your results, then what good is the test? Keep at it until you achieve the results you want. I did. And this is the best I’ve felt in many years. You can too.
[vii] Hinz M., Stein A., Uncini T. “Validity of urinary monoamine assay sales under the “spot baseline urinary neurotransmitter testing marketing model”” International Journal of Nephrology and Renovascular Disease 2011; 4: 101–113. (source)
[viii] Bjelland I, Tell GS, Vollset SE, Refsum H, Ueland PM. “Folate, vitamin B12, homocysteine, and the MTHFR 677CT polymorphism in anxiety and depression.” The Hordaland Homocysteine Study. Archives of General Psychiatry. 2003 Jun;60(6):618-26. (source)
[xi] Kiecolt-Glaser JK, Preacher KJ, MacCallum RC, Atkinson C, Malarkey WB, Glaser R. “Chronic stress and age-related increases in the proinflammatory cytokine IL-6.” Proceedings of the National Academy of Sciences. 2003 Jul 22;100(15):9090-5. (source)
[xii] Lee J.M., Okumura M.J., Davis M.M., Herman W.H., Gurney J.G. “Prevalence and determinants of insulin resistance among U.S. adolescents: a population-based study.” Diabetes Care. 2006 Nov;29(11):2427-32. (source)
[xiii] Kleinridders A. “Insulin resistance in brain alters dopamine turnover and causes behavioral disorders” Proceedings of the National Academy of Sciences of the United States of America vol. 112 no. 11 (source)
[xiv] Werner H., LeRoith D. “Insulin and insulin-like growth factor receptors in the brain: Physiological and pathological aspects” European Neuropsychopharmacology Volume 24, Issue 12, Pages 1947–1953 (source)
[xv] Di Marzio L., Moretti S., D'Alò S., Zazzeroni F., Marcellini S., Smacchia C., Alesse E., Cifone M.G., De Simone C. “Acetyl-L-carnitine administration increases insulin-like growth factor 1 levels in asymptomatic HIV-1-infected subjects: correlation with its suppressive effect on lymphocyte apoptosis and ceramide generation.” Clinical Immunology. 1999 Jul;92(1):103-10. (source)
[xviii] Pollock M.A., Sturrock A., Marshall K., Davidson K.M., Kelly C.J., McMahon A.D., McLaren E.H. “Thyroxine treatment in patients with symptoms of hypothyroidism but thyroid function tests within the reference range: randomised double blind placebo controlled crossover trial.” BMJ. 2001 Oct 20;323(7318):891-5. (source)