Methylene Blue (methylthioninium chloride) is a synthetic compound used as a nootropic to increase memory, mood and longevity.
Soon after Methylene Blue was synthesized as a textile dye in the late 1800’s, it became the first synthetic drug to be used in humans. It was used for the treatment of malaria.
In the early 20th century, psychiatrists were using Methylene Blue in the experimental treatment of schizophrenia.[i]
Methylene Blue is currently being studied as potential therapy for mild cognitive impairment, Alzheimer’s and Parkinson’s disease, and other neurodegenerative disorders. All sharing a common problem with mitochondrial function.
Your brain’s neurons rely almost entirely on mitochondria-derived energy. Failure of mitochondrial function can affect the rest of your body. But it’s particularly detrimental to your brain.
This is where Methylene Blue steps in as possibly one of the most important anti-aging and neurological disease preventing nootropics we have available today.
As a nootropic, Methylene Blue quickly crosses the blood-brain barrier. It improves mitochondrial efficiency and respiration, acts as an antioxidant, and increases brain cell lifespan. Resulting in improved memory and mood.
Here we’ll dive into over 140 years of research on how Methylene Blue helps your brain.
Methylene Blue helps:
- Neurotransmitters: Methylene Blue inhibits monoamine oxidase and acetylcholinesterase activity which increases levels of catecholamines and acetylcholine. And boosts serotonin and norepinephrine affecting anxiety, depression and memory.
- Mitochondrial Energy: Methylene Blue assists brain cell respiration by increasing oxygen. And donating electrons to the electron transport chain within mitochondria. This same process is used to create ATP within mitochondria from the food you eat. So MB contributes to this energy-production process in place of the nutrients you get from your food. Increasing cellular energy positively effects mood and memory.
- Neuroprotectant: Methylene Blue is a potent antioxidant. Reactive oxygen species are produced inside mitochondria. The first free radical that is formed inside a cell is superoxide. MB will bind to superoxide and reduce it to water. It stops the oxidative cascade at its very beginning. Before it gets a chance to do damage.
Table of Contents
Overview
Methylene Blue (methylthioninium chloride) was first synthesized in 1876 by German chemist Heinrich Caro at BASF as an aniline-based dye for cotton staining.
In 1891, German physician and Noble Prize recipient Paul Ehrlich pioneered the use of Methylene Blue for the treatment of malaria.[ii]
Ehrlich discovered that when MB was injected into animals in the lab, it would quickly concentrate in the brain. And had an uncanny ability to selectively target diseased tissues in the body.
It was Ehrlich who coined the term “Magic Bullet” for this unique action displayed by Methylene Blue. A term still in use today.
Methylene Blue has since been used to treat dementia, in cancer chemotherapy, malaria, methemoglobinemia, urinary tract infections, cyanide and carbon monoxide poisoning.[iii]
As a nootropic, Methylene Blue is used to enhance mitochondrial function, increase cerebral blood flow, and acts as an antidepressant.
How does Methylene Blue work in the brain?
Methylene Blue boosts brain health and function in several ways. But two in particular stand out.
- Methylene Blue improves memory. Unlike other nootropics which often work by increasing neurotransmitter synthesis and neural signaling, MB improves memory by increasing brain cell respiration. Or how the brain cell utilizes oxygen.
Studies show dramatic increases of cellular oxygen consumption and glucose uptake when using Methylene Blue. MB increases CMRO2 (cerebral metabolic rate) through increased activity in the mitochondrial electron transport chain.
Methylene Blue functions as an alternative electron carrier in the electron transport chain in mitochondria. It accepts electrons from NADH and transfers them to cytochrome c.[iv]
Cytochrome complex (cytochrome c) is a component of the electron transport chain in mitochondria. Playing a role in apoptosis and as an antioxidant.
Methylene Blue also stimulates glucose metabolism. Taken together, increases in CMRO2 and glucose uptake means that MB elevates oxygen consumption which helps glucose increase ATP production.
Increases in ATP production provides more cellular energy for better overall brain function including cognition, mood and memory.
- Methylene Blue is an antioxidant. MB has a unique mechanism of action that is fundamentally different from traditional antioxidants. During cellular respiration, the first free radical formed inside a cell is superoxide (O2).
Methylene Blue binds to superoxide and reduces it to water. It stops the oxidative cascade at its very beginning. Before it gets a chance to do damage.[v]
So think of Methylene Blue as having a unique dual property. First, it increases cellular energy production which normally leads to oxidative stress. And second, it eliminates this oxidative stress. Making it a metabolic enhancer and an antioxidant.
Researchers tested Methylene Blue in animal models of neurological disease. First, researchers used rotenone (a potent pesticide) which causes severe dopamine depletion in the part of the brain associated with Parkinson’s.
Methylene Blue rescued brain cell mitochondria from the damaging effects of this toxin. By donating electrons in the electron transport chain broken by rotenone. Essentially bypassing the broken transport chain with donated electrons as an alternative electron carrier.
Methylene Blue also countered cerebral ischemia reperfusion damage. The tissue damage caused when blood supply returns to tissue after a lack of oxygen from a stroke. And can occur with Traumatic Brain Injury. MB accomplished this by rerouting mitochondrial electron transfer.
And Methylene Blue dramatically countered the behavioral, neurochemical, and neuropathological impairment found in Parkinson’s disease.[vi]
How things go bad
As we live life, our brain chemistry and metabolism changes.
↓ Mitochondrial energy levels decline
↓ Attention, memory and mental agility decline
↓ Tau proteins and amyloid plaques clog the brain
↓ Free radicals damage brain cell mitochondria
↓ Cerebral blood flow declines
All of these changes can happen at any age. And are a product of the food we eat, what we drink, lifestyle habits, the air we breathe and more.
So Methylene Blue can help for age-related cognitive decline, as well as a student looking to do better in school. By boosting brain cell mitochondria energy production levels. And improving cerebral blood flow.
Methylene Blue benefits
Low dose Methylene Blue supplementation provides memory enhancing effects in animals and humans. It works as an antidepressant, is anti-aging, helps dementia, Huntington’s and Alzheimer’s.
Methylene Blue increases low blood pressure, improves cognition in healthy people, boosts mitochondrial function, is anti-microbial, can help eliminate fear and even slow skin aging.
Methylene Blue boosts acetylcholine
Research shows that Methylene Blue is an acetylcholinesterase inhibitor with a preference for muscarinic acetylcholine receptors. Meaning MB prevents the breakdown of acetylcholine and making more available in your brain.[vii]
Methylene Blue is an antidepressant
Methylene Blue is a monoamine oxidase inhibitor (MAOI). It inhibits MAO-A more than MAO-B, but inhibits both at large doses.[viii]
One study in 1987 showed that 15 mg per day of Methylene Blue was a potent antidepressant in those with severe depression.[ix]
Another study with 31 Bipolar Disorder patients compared 300 mg per day of Methylene Blue with 15 mg per day. The patients were also on lithium treatment. The study showed that the 300 mg dose of Methylene Blue was a “useful addition to lithium in the long-term treatment of manic-depressive psychosis”. And patients were significantly less depressed.[x]
Methylene Blue resists Alzheimer’s Disease
Alzheimer’s disease and other forms of dementia are associated with a buildup of the protein Tau. Clinical trials show that Methylene Blue inhibits Tau formation. And is under consideration as a treatment for Alzheimer’s.[xi]
Methylene Blue has an inhibitory action on the cGMP pathway, and affects other molecular events closely related to the progression of Alzheimer’s.
Methylene Blue boosts neuron resistance to the formation of amyloid plaques and neurofibrillary tangles. And helps repair impairments in mitochondrial function and cellular metabolism.
Research also shows that cholinergic, serotonergic and glutamatergic systems all play important roles in the development of Alzheimer’s and other cognitive disorders. Methylene Blue provides beneficial effects in mediating these pathways.[xii]
This is particularly significant because most existing treatments for Alzheimer’s can only prevent the disease before it is diagnosed. But Methylene Blue shows promise in delaying the effects of Alzheimer’s and dementia after it is diagnosed.
Methylene Blue is anti-aging
Research shows that Methylene Blue is an effective anti-aging nootropic. MB increases mitochondrial complex IV by 30%, enhances cellular oxygen consumption by 37-70%, increases heme synthesis, and reverses premature senescence caused by H2O2 or cadmium.
Methylene Blue is considered a redox agent. Meaning it cycles between oxidized and reduced forms. This cycling by MB helps block oxidant production in brain cell mitochondria.[xiii]
Mitochondrial complex IV is the last enzyme in the respiratory electron transport chain in mitochondria. The last step in synthesizing ATP. Your cellular source of energy.
Iron (heme) is an essential element and participates in oxygen transport, DNA synthesis and electron transport. Heme synthesis begins in mitochondria. Every cell requires heme to function properly.[xiv]
Senescence or biological aging is the gradual deterioration of cellular function. And is caused by telomere shortening that triggers DNA damage in response to reactive oxygen species, hydrogen peroxide (H2O2), cadmium and other toxins. Methylene Blue helps prevent premature senescence or premature cell death.
Methylene Blue improves memory
Animal studies have shown that a single low dose of Methylene Blue enhances long-term contextual memory. This type of memory is the conscious recall of the source and circumstances of a specific memory.
Other studies show that Methylene Blue in low doses taken after the event helps memory retention of the event. A study done with rats revealed why this works.
In this study, rats received 1 mg/kg of MB post-training for 3 days. The researchers then measured cytochrome c oxidation in participants brains. The idea was to determine if an increase in metabolic energy was behind the memory enhancing qualities of MB.
The study found that in the Methylene Blue treated group, brain cytochrome oxidase activity was 70% higher than in the placebo-treated group.
The findings suggest that repeated post-training supplementation of Methylene Blue improves memory consolidation. And this memory boost is due to the increased metabolic capacity in brain regions that require more energy during discrimination learning.[xv]
How does Methylene Blue feel?
Methylene Blue as a nootropic will likely feel different than any other supplement you’ve ever tried.
When reading or studying, once you’re done, you should feel like you fully understand the subject material. And you’ll be able to use what you learned in the future.
Methylene Blue seems to facilitate a full understanding of something on the first try.
Many neurohackers report the biggest nootropic effect they experience with Methylene Blue is “after the fact learning”. You take in the information. And it’s like your brain sorts through the material. Then stores it in a form you can easily access later.
Methylene Blue has this uncanny ability to rewire your brain to forget about any negative associations you have of a situation. And only retains the positive aspects of that memory.
Some report that Methylene Blue makes you “feel young again”. It eliminates social anxiety. You’ll feel focused and more confident.
Workouts seem easier because you have more energy. Your mitochondria are energized. And you may find that recovery from workouts is easier.
Methylene Blue helps eliminate stress. So you have more energy in any situation with a relaxed state of mind.
And one recurring theme from many is improved sleep while using Methylene Blue.
Methylene Blue Clinical Research
Research into Methylene Blue for its therapeutic value goes back to the late 1800’s. But it’s only in the last couple of decades that scientists have been able to decode exactly how Methylene Blue works in the brain. All the way down to the molecular level in mitochondria.
A study in 2017 found that Alzheimer’s Disease could be caused by more than the accumulation of amyloid beta in the brain. The scientists looked into the two main components that produce energy in cells.
- Glycolysis is the mechanism used to convert glucose into fuel within mitochondria.
- Creating this fuel in mitochondria uses oxygen in a process called mitochondrial respiration.
The researchers found that as the brain ages, mitochondrial metabolism deteriorates. Resulting in a reduction in the molecules needed for energy production. And possibly the main culprit behind many neurological diseases including Alzheimer’s and Parkinson’s.[xvi]
A study conducted at Children’s Hospital Oakland Research Institute may have found the solution to this mitochondrial energy problem in brain cells.
The researchers found that Methylene Blue can prevent or slow the decline of mitochondrial function.
One of the key aspects of Alzheimer’s is mitochondrial dysfunction. Specifically complex IV dysfunction. And this is where Methylene Blue steps in.
The study found that Methylene Blue enhances complex IV in mitochondria. It increases oxygen consumption. And it reverses premature cell death.
The researchers concluded that Methylene Blue may be useful to delay mitochondrial dysfunction with aging and the decrease in complex IV in Alzheimer’s disease.[xvii]
Methylene Blue Improves Memory
26 healthy volunteers aged 22 – 62 participated in a double-blind, randomized, placebo-controlled clinical trial. Purpose of the study was to measure the effects of Methylene Blue on working memory and sustained attention.
Study results showed that a single low-dose of Methylene Blue resulted in an increase in short-term memory ability. In an area of the brain associated with the senses and visual processing.
Timothy Duong, Ph.D., the study author concluded Methylene Blue showed promise “in healthy aging, cognitive impairment, dementia and other conditions that might benefit from drug-induced memory enhancement”.[xviii]
Another study published in the American Journal of Psychiatry tested fear extinction (elimination of fear) and contextual memory. Both are forms of long-term memory.
In this study, participants received either Methylene Blue or a placebo after being placed in a small dark chamber for a couple of minutes to address their fear.
One month later, participants that had used Methylene Blue had less retained fear than those given a placebo. The study authors concluded, “Methylene Blue enhances memory and the retention of fear extinction”.[xix]
Methylene Blue helps Bipolar Disorder
Increasing evidence shows that Bipolar Disorder may be a progressive condition. Dealing with bipolar symptoms long-term can result in deterioration of the cortex and less gray matter.
A study at Dalhousie University in Halifax compared the effectiveness of lamotrigine (Lamictal®) along with daily supplementation of either 195 mg or 15 mg of Methylene Blue in patients with Bipolar Disorder.
Patients took a dose (Lamictal and either 195 mg or 15 mg of Methylene Blue) for three months. Then switched to the other dose for 3 months. The team found that bipolar participants had reduced depression and anxiety when taking the higher dose of Methylene Blue compared to when they were on the lower dose.
Several Bipolar Disorder patients in the study chose to continue using Methylene Blue after the study concluded.[xx]
Methylene Blue Recommended Dosage
Recommended safe doses based on clinical studies with animals and humans ranges from 0.5 – 4 mg/kg. So a 90 kg (200 lb.) body weight translates to 45 – 360 mg of Methylene Blue.
45 mg of Methylene Blue is a safe starting dose. But 360 mg of MB is much too high in my opinion even if you’re 200 lbs.
The bottom line is there is no true recommended dose for Methylene Blue. My recommendation is start with the lowest dose of 0.5 mg/kg and see how you react.
Methylene Blue is water-soluble so you don’t need to take it with a meal, or healthy fat like some nootropics.
Methylene Blue has a half-life of about 5 hours. So you can dose twice a day.
Methylene Blue is famous for turning urine blue. But for most neurohackers, your urine will only stain blue at doses roughly exceeding 500 mcg. Some recommend preventing blue urine by mixing Methylene Blue with ascorbic acid for 3 hours before taking it.
Methylene Blue is also available as a doctor-administered injection for therapeutic use. Typically used to treat diseases like malaria or with anti-cancer therapy.
Methylene Blue Side Effects
Methylene Blue shows a hormetic dose-response, with opposite effects at low and high doses.
In other words, lower doses of Methylene Blue work well as a nootropic. But high doses do not because MB can potentially “steal” electrons away from the electron transport chain. Disrupting the redox balance and acting as a pro-oxidant (instead of an antioxidant).[xxi]
Adverse effects of Methylene Blue also come from chemical impurity. Even pharmaceutical (USP) grade Methylene Blue can contain impurities like arsenic, aluminum, cadmium, mercury and lead.
At low doses, these contaminants are not that big of a problem. But higher doses will result in the accumulation of these toxins in your cells.
Side effects with Methylene Blue are rare when doses are under 2 mg/kg. But can include stomach pain, chest pain, dizziness, headache, sweating, confusion, high blood pressure, shortness of breath, accelerated heartbeat, tremor, skin turning blue, urine turning blue or green, reduction of red blood cells, or jaundice (only reported in infants).
Monoamine Oxidase (MAOI) inhibition becomes a big problem at around 2 mg/kg of Methylene Blue. So if you are using antidepressants or antianxiety medications that affect dopamine or serotonin, you should NOT use Methylene Blue. Because using Methylene Blue with one of these medications could cause a hypertensive crisis or Serotonin Syndrome.
This includes SSRI’s and MAOI’s such as citalopram, escitalopram, fluoxetine, fluvoxamine, paroxetine, sertraline, zimelidine, bupropion, buspirone, clomipramine, mirtazapine and venlafaxine.
Do not use Methylene Blue if you are pregnant or breast-feeding.
Where to buy Methylene Blue
Methylene Blue is sold as a liquid, and in crystalline powder form.
Industrial-grade and chemical-grade Methylene Blue is sold as a dye or stain. And can consist of more than 8% – 11% of various contaminants. And should NOT be used as a nootropic.
Only pharmaceutical (USP) grade Methylene Blue should be used as a nootropic. Ask for a Certificate of Analysis which should include the amount of contaminants such as arsenic, aluminum, cadmium, mercury and lead.
I recommend CZTL Methylene Blue due to their robust testing program. They ship in 1 gram containers of powder with dosage and mixing instructions in each shipment. Along with a Certificate of Analysis (CofA) verifying the purity of the Methylene Blue you get from them.
Nootropics Expert Recommendation
Methylene Blue 0.5 – 4 mg/kg of body weight per day
I recommend using Methylene Blue as a nootropic supplement.
Your body does not make Methylene Blue on its own. So to get its benefits you must take it as a supplement.
Methylene Blue is especially helpful for those dealing with anxiety and depression.
Methylene Blue is also particularly useful to students and executives who want to boost cognition, learning and memory.
Methylene Blue is a fast-acting nootropic that can also help prevent brain mitochondrial degeneration. Providing potential as an anti-aging nootropic.
Methylene Blue donates electrons in the electron transport chain in your mitochondria. So experienced neurohackers suggest avoiding CoQ-10 or idebenone when using MB because it seems to make Methylene Blue much less effective. Remember earlier in this review when I said the too much Methylene Blue can be counter-productive. The same applies here.
Idebenone is an Alzheimer’s drug that has some nootropic benefit. But it works similar to Methylene Blue because it acts as a transporter in the electron transport chain in mitochondria. Possibly potentiating Methylene Blue.
I recommend CZTL Methylene Blue
[i] Allexsaht W.J. “The use of methylene blue in the treatment of catatonic dementia praecox patients.” Psychiatric Quarterly. 1938;12:245–252.
[ii] Schirmer R.H., Coulibaly B., Stich A., Scheiwein M., Merkle H., Eubel J., Becker K., Becher H., Müller O., Zich T., Schiek W., Kouyaté B. “Methylene blue as an antimalarial agent.” Redox Report. 2003;8(5):272-5. (source)
[iii] Wainwright M., Crossley K.B. “Methylene Blue--a therapeutic dye for all seasons?” Journal of Chemotherapy 2002 Oct;14(5):431-43. (source)
[iv] Wen Y., Li W., Poteet E.C., Xie L., Tan C., Yan L.J., Ju X., Liu R., Qian H., Marvin M.A., Goldberg M.S., She H., Mao Z., Simpkins J.W., Yang S.H. “Alternative mitochondrial electron transfer as a novel strategy for neuroprotection.” Journal of Biological Chemistry. 2011 May 6; 286(18):16504-15. (source)
[v] Poteet E. et. Al. “Neuroprotective Actions of Methylene Blue and Its Derivatives” PLOS One 2012; 7(10): e48279. (source)
[vi] Wen Y., Li W., Poteet E.C., Xie L., Tan C., Yan L.J., Ju X., Liu R., Qian H., Marvin M.A., Goldberg M.S., She H., Mao Z., Simpkins J.W., Yang S.H. “Alternative mitochondrial electron transfer as a novel strategy for neuroprotection.” Journal of Biological Chemistry. 2011 May 6; 286(18):16504-15. (source)
[vii] Pfaffendorf M., Bruning T.A., Batnik H.D., van Zwieten P.A. “The interaction between methylene blue and the cholinergic system.” British Journal of Pharmacology. 1997 Sep;122(1):95-8. (source)
[viii] Ramsay R.R., Dunford C., Gillman P.K. “Methylene blue and serotonin toxicity: inhibition of monoamine oxidase A (MAO A) confirms a theoretical prediction.” British Journal of Pharmacology. 2007 Nov;152(6):946-51 (source)
[ix] Naylor G.J., Smith A.H., Connelly P. “A controlled trial of methylene blue in severe depressive illness.” Biological Psychiatry. 1987 May;22(5):657-9. (source)
[x] Naylor G.J., Martin B., Hopwood S.E., Watson Y. “A two-year double-blind crossover trial of the prophylactic effect of methylene blue in manic-depressive psychosis.” Biological Psychiatry. 1986 Aug;21(10):915-20. (source)
[xi] Crowe A., James M.J., Lee V.M., Smith A.B. 3rd, Trojanowski J.Q., Ballatore C., Brunden K.R. “Aminothienopyridazines and methylene blue affect Tau fibrillization via cysteine oxidation.” Journal of Biological Chemistry. 2013 Apr 19;288(16):11024-37 (source)
[xii] Oz M., Lorke D.E., Petroianu G.A. “Methylene blue and Alzheimer's disease.” Biochemical Pharmacology. 2009 Oct 15;78(8):927-32. (source)
[xiii] Atamna H., Nguyen A., Schultz C., Boyle K., Newberry J., Kato H., Ames B.N. “Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways.” FASEB Journal. 2008 Mar;22(3):703-12. (source)
[xiv] Abbaspour N., Hurrell R., Kelishadi R. “Review on iron and its importance for human health” Journal of Research in Medical Sciences 2014 Feb; 19(2): 164–174. (source)
[xv] Wrubel K.M. et. El. “The Brain Metabolic Enhancer Methylene Blue Improves Discrimination Learning in Rats” Pharmacological and Biochemical Behavior. 2007 Apr; 86(4): 712–717. (source)
[xvi] Sonntag K.C., Ryu W., Amirault K.M., Healy R.A., Siegel A.J., McPhie D.L., Forester B., Cohen B.M. “Late-onset Alzheimer’s disease is associated with inherent changes in bioenergetics profiles” Scientific Reports 2017; 7 (source)
[xvii] Atamna H., Nguyen A., Schultz C., Boyle K., Newberry J., Kato H., Ames B.N. “Methylene blue delays cellular senescence and enhances key mitochondrial biochemical pathways.” FASEB Journal. 2008 Mar;22(3):703-12 (source)
[xviii] Rodriguez P., Zhou W., Barrett D.W., Altmeyer W., Gutierrez J.E., Li J., Lancaster J.L., Gonzalez-Lima F., Duong T.Q. “Multimodal Randomized Functional MR Imaging of the Effects of Methylene Blue in the Human Brain.” Radiology. 2016 Nov;281(2):516-526. (source)
[xix] Telch M.J., BRuchey A.K., Rosenfield D., Cobb A.R., Smits J., Pahl S., Gonzalez-Lima F. “Effects of Post-Session Administration of Methylene Blue on Fear Extinction and Contextual Memory in Adults With Claustrophobia” American Journal of Psychiatry Volume 171, Issue 10, October 2014, pp. 1091-1098 (source)
[xx] Alda M., McKinnon M., Blagdon R., Garnham J., MacLellan S., O'Donovan C., Hajek T., Nair C., Dursun S., MacQueen G. “Methylene blue treatment for residual symptoms of bipolar disorder: randomised crossover study.” British Journal of Psychiatry. 2017 Jan;210(1):54-60 (source)
[xxi] Rojas J.C., Bruchey A.K., Gonzalez-Lima F. “Neurometabolic mechanisms for memory enhancement and neuroprotection of methylene blue” Progress in Neurobiology. 2012 Jan; 96(1): 32–45. (source)
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Any tips or advice for mthfr/c677t? I do not have the blood issue. I have been doing very slow for a couple of weeks and I am wondering if its helping me more with detox? Any thoughts
Melanie, read through all the benefits of Methylene Blue in the above review. That is everything that it’s doing in your body and brain.
David, I am anxious to start an MB regimen. I have the product on hand, mixed 1 mg to 100 ml distilled water. How do I take this, and will it stain my mouth and teeth? That might be an improvement….LOL!
John, CZTL has a chart on their FAQ page telling you how many mg in each drop of Methylene Blue depending on solution strength. Anything up to 45 mg per day is considered safe. Many find lower dosages work as well. You’ll need to figure out what works for you through experimenting.
And it will stain your tongue teeth, and urine blue. It is fundamentally a stain and will stain anything it comes into contact with.
Want to put Methylene Blue and Melatonin in my emergency kit for Stroke possibilities but cannot find anywhere through searching how much or how to give to person. Read articles both of these would be excellent but no details.
Julie, according to this study (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4817094/) the stroke patients were given from 2 – 5 mg/kg of Methylene Blue.
And according to this study (https://www.ahajournals.org/doi/full/10.1161/01.str.0000057460.14810.3e) it was 5 mg/kg of Melatonin that worked ONLY IF it was taken within one hour of the stroke. If so, then subsequent doses also helped. But the first dose was key to making this work.
Can Reagent grade MB be safely consumed instead of USP grade, even if it has not been tested as a consumable?
Vernon, no, reagent Methylene Blue cannot be safely used as a nootropic. It certainly is NOT pharmaceutical grade.
Hi David
I have bought CZTL methylene blue and have been using it at a 1% concentration at 60 drops per day. It has been amazing for me – I have Crohn’s disease and all symptoms such as diarrhea/fatigue have stopped. My question is; I have been taking it for two months and last week I ran out. My new shipment is still to arrive and my symptoms have started coming back again 🙁
Would a higher dose work to really stunt symptoms so I can spend a greater length of time off it in future (wanting to get pregnant next year and won’t be able to take it then /but wanting to be as healthy as possible before then if that makes sense!)
Thanks!
Annie, you can NOT use a higher dose of Methylene Blue than recommended because MB can potentially “steal” electrons away from the electron transport chain. Disrupting the redox balance and acting as a pro-oxidant (instead of an antioxidant).
Great info, David,
You wrote about dosage, yet a bit more practical would be great for me and perhaps other readers.
Say I made a mix of one gram into 100 ml of water. How many drops would be good for an average weight man/woman?
Thank you,
Maria
Maria, your question is difficult to answer because everyone responds differently to these nootropics. I can say that on average 45 mg MB per day works but that may be way too much for you.
Use the chart that CZTL has in this page: https://cztl.bz/pages/commonly-asked-questions-about-methylene-blue to figure out your dosage. Then start experimenting starting at 10 mg per day. And increase it by 10 mg the next day. Keep raising it until you hit your sweet spot.
But also keep in mind that too much Methylene Blue is bad because it starts working as a “prooxidant” instead of an antioxidant. Causing more damage than what you started with.
What are possible complications of using MB if you have slow COMT issues from several genetic mutations and tend to get over methylated? Possible side effects? Is it’s use contraindicated?
Thanks for your input!
Laura, it’s not possible to know if Methylene Blue is contraindicated for you unless you try it. Everyone is different and there are so many permutations and combinations when it comes to how we are structured that it’s not possible to predict how any one person will react to a supplement. Unless it is contraindicated with any meds they are using.
Thank you for this very informative website. I just started taking methyline blue today. I also take regular supplements like multi vitamin. The one supplement i am concerned about are essential amino acids. I don’t see anything about it in drugs.com. Do you have any thoughts on this? Thank you, Marcus
Marcus, I do not know what you mean by “essential amino acids”. Because there a lot of them. It depends on your age and what you may be deficient in.
LDN is a different kind of “medicine” for it is not really a “medicine”, so want to make sure you know that when saying MB is not contradicted with LDN.
The basic effects of LDN can be summarized as follows:
DEXTRO-Naltrexone
Blocks (antagonises) some TLR receptors
Reduces production of pro-inflammatory cytokines
Suppresses cascade inflammation
Central and system effects as TLR receptors are present on microglial cells, mast cells, and macrophages
LEVO-Naltrexone
Blocks opiate receptors for a brief period
Increases natural production of anti-inflammatory endorphins
Upregulates opiate receptors
Has direct effect on some cell proliferation rates
P.S. I am the person who often gets paradoxical (the opposite of what it intended to achieve) effects.
Sam, that my be so but drugs.com is pretty good about including things that are not really ‘drugs’ including some natural nootropic supplements when appropriate.
Hi David,
I’m delighted to have stumbled on your article during my search. Great page with lots of information so I’ll be spending some time here!
I have hashimotis and take synthetic t4 and t3 as well as NAC and LDN (low dose naltrexone). I do take a few other supplements but I’ll check those against the list provided in the link above. My concern is specifically these 4 that I’ve listed.
Thank you for your time.
Kindly
Launa
Launa, the only supplements that are contraindicate with thyroid meds are Ashwagandha, Lemon Balm extract and ALCAR (for some people). Those will mess with your thyroid and should be avoided.
Hello,
I take Low Dosage Naltrexone daily. Is it okay to take MB?
Thank you.
Sam, Low Dose Naltrexone is not on the contraindicated list here: https://www.drugs.com/drug-interactions/methylene-blue-index.html
Thank you
Is it OK to take Methylene Blue if one is on
1- Mucuna pruriens (L-Dopa) 2- 5-HTP and 3- Tyrosine ?
My concern is that Methylene Blue being a potent MAO inhibitor, would it not lead to perturbations of 5-hydroxytryptamine metabolism ?
What is your take on that ?
This is regarding someone not taking any drug whatsoever, not even a single one, but who is regularly taking those 3 supplements.
Would it then be safe to take Methylene Blue along with those 3 supplements above listed?
My concern is safety, and would it compromise the efficacy of those 3 above listed supplements? I am
referring to this study – link below – that has raised my concern:
https://pubmed.ncbi.nlm.nih.gov/17721552/
Sabine, this is a list of the ‘drugs’ that are contraindicated with Methylene Blue: https://www.drugs.com/drug-interactions/methylene-blue-index.html
And you will notice that all 3 supplements that you are currently using are highly contraindicated. Count L-Tyrosine the same as you would L-DOPA.
Thank You for confirming that,
I do not take those 3 supplements, someone around me does,
I am happy to have you confirming and to see from the link you sent – very helpful link indeed- that the Methylene Blue usage will definitely need to be postponed until AFTER those supplements regimen is no longer implemented !
I am very grateful for this advice and the link !
Hey there. Have you ever heard about anyone experiencing dry/irritated eye(s) the morning after a day in which methylene blue is used?
Verified now after multiple instances.
>> I jabbed my eye in March’22 and its been on/off sensitive to light and dry conditions since. I hit the corner of the eye socket, not the actual eye but I was unable to open that eye for almost 2 days. If I use more than 0.5mg/kg of methylene blue in a day, that eye is dry and irritated for a an hour or two when I wake up the next day. It gets more intensely dry as the dose increases
Mostly just leaving this here for others, and maybe you find it interesting. Im not expecting, nor actively seeking out a solution.
Ryan, not a side effect I was aware of. Thanks for sharing.
Hi David,
Thanks for all your info on this subject. Just wondering if you’re positive that CZTL is a safe brand? I’ve seen mixed reviews. It is so much cheaper than any liquid forms I’ve looked at and am hoping it’s not too good to be true! Thanks in advance
Nathan, I’ve only seen great reviews for CZTL. I have not received a single complaint about recommending them.
How far away from CoQ10? (hours?)
Alcohol? If I do MB at noon is it safe to have a drink at 5?
Just started 2 days ago and when it kicks in it is quite amazing. I feel energized, focused and content (i.e. irritability is gone). I have gotten more work done recently than in weeks prior.
Ann I have already commented on both of those in this thread. Please search through the comments to see my answers.
I did see the references. From what I saw mixing alcohol and MB causes potential death. Point taken and thx for the warning. What I didn’t see was when it would be safe – what time frame between the 2. Same for CoQ10. I like to chew mine so it enters the gum tissue – its good for oral health. So might it be ok to do CoQ10 at midnight if I do MB at noon?
Ann, that should work out because Methylene Blue is used quickly by your system.
When ordering from that site, if I get 1 gram how much water do I mix it with? Then how many drops would equal how many milligrams?
Thanks!
Angie, they have a page on their website with instructions on how to create different strength of solution.
Is it O.K. to take MB with DMSO.
I know of an individual who has gradually lost the ability to walk. They can draw the knees to the chest and lift them from a sitting or horizontal position, mind you with difficulty, but they will not support, to even use a walker
If there was such a thing as a brain tumor, would MB reduce it?
Thanks,
Ellen
Ellen, I know nothing about Dimethyl sulfoxide (DMSO) so cannot comment on if it is contraindicated with Methylene Blue. And as far as I can tell Methylene Blue will not likely have any effect on a brain tumor.
@Connie,
Were they checked for Paraneoplastic Syndrome?
Hi,
DMSO acts as an intensifier, anti-inflammatory, anti-oxidant and more.
If you are interested in using DMSO for Tumor try it with an Artemisia Annua Extract with 99% DMSO.
check the scientific studies around AA in combination with tumors online.
Best wishes,
V
Is drinking alcohol contraindicated with MB? Even in small units?
Robert, Google this: “What happens when you mix Methylene Blue and alcohol”. I do not think you’ll like the results. Because they include coma and death.
I put that search string and found only one site which was a dependency site https://www.worldsbest.rehab/methylene-blue-and-alcohol/ which warned of consequences, Is there perhaps more corroboration of this issue. i.e. quantities, simultaneous consumption. Time between each. Thanks Greg
Greg, keep on scrolling through your search results and you will find the clinical proof you are looking for.
Hi, I would share my experience with Methylene Blue (MB).
For me, the best feeling is when I dose this about 15-20 mg range. If I take 45 mg, it makes me dizzy and confused (possibly due to surge of serotonin(?)).
I do not use any medications. Rarely I take 300 mg of Phenibut before intensive studying (but the confusion shows even if I do not take the Phenibut together with MB). Any thoughts on this?
Also, I take MB for 2 months now. Not sure I feel the learning benefits so far. I feel similar high to other drugs that effects dopamine and serotonin pathways, but nothing else.
I also would like to share my thoughts about mixing MB with vitamin C (so called: leuco-Methylene Blue), I noticed that taking regular MB feels better than leuco-MB. It is possible that too much Ascorbic Acid (2-3g with MB to make it colorless) could also act as an pro-oxidant and would not be beneficial.
Westley, Phenibut takes up to 4 hours to fully take effect which may account for the experience you mentioned.
And your last comment on ascorbic acid makes logical sense. You could be on to something that I hadn’t thought of. Thanks.
Hello, I am just getting started on my MB journey to assist with brain fog and energy related to peri-menopause. I am also on low dose, bio-identical estrogen for one week a month, prior to my cycle. Is this counter intuitive?
I am also curios if there are any interactions with ozone (insufflation or IV)
Is it ok to have a glass of wine on occasion with MB?
And one more, Quercetin. Is there a doubling effect (thinking zinc uptake) with taking both?
Thanks so much for your wealth of info in support of health!
Cristin, the only thing you want to be very careful of is combining alcohol with Methylene Blue. That could put you into a coma or even kill you.
Sorry to keep responding to all of these.
But the source people here are getting about mixing MB and alcohol causing death is from a fake AI generated article.
Actual studies show mixing MB with alcohol is harmless.
https://academic.oup.com/alcalc/article/35/5/424/206576
Mike, that study concludes “no effect of methylene blue on the disposition of ethanol and its metabolic consequences could be demonstrated in humans, possibly because the dose of methylene blue that can be safely administered to humans is too low to be effective.”
Do you really want to take the chance of mixing the two? If so, then go for it. But I would not risk it.
Why would you not risk it?
Your basing your views on this issue on an AI generated article that is completely made up from nothing.
The actual studies on this subject, the one I posted for example say no effect. It’s extremely likely to kill you and I see no reason why this would be the case.
Mike, you are making a lot of unwarranted assumptions here. It is entirely up to you if you want to use something. I cannot and will not take take on that responsibility legally or morally.